Camouflaged cell-membrane-based nanoparticles have gained increasing attention owing to their improved biocompatibility and immunomodulatory properties. Using nanoparticles prepared from the membranes of specific cell types or fusions derived from different cells membranes, their functional performance could be improved in several aspects. Here, cell membranes extracted from breast cancer cells and platelets are used to fabricate a hybrid-membrane vesicle (cancer cell-platelet-fusion-membrane vesicle, CPMV) loaded with therapeutic microRNAs (miRNAs) for the treatment of triple-negative breast cancer (TNBC). A clinically scalable microfluidic platform is presented for fusion of cell membranes. The reconstitution process during synthesis allows for efficient loading of miRNAs into CPMVs. Conditions for preparation of miRNA-loaded CPMVs are systematically optimized and their property of homing to source cells is demonstrated using in vitro experiments and therapeutic evaluation in vivo. In vitro, the CPMVs exhibit significant recognition of their source cells and avoided engulfment by macrophages. After systemic delivery in mice, CPMVs show a prolonged circulation time and site-specific accumulation at implanted TNBC-xenografts. The delivered antimiRNAs are sensitized TNBCs to doxorubicin, resulting in an improved therapeutic response and survival rate. This strategy has considerable potential for clinical translation to improve personalized therapy for breast cancer and other malignancies. 相似文献
The biostratigraphy, microfacies association, mineralogical interferences, depositional environments and geochemical properties of the Abu Qada Formation, which exposed at Gabal El-Gunna Southeastern Sinai, Egypt were delineated. It belongs to the planktonic foraminiferal Whiteinella archaeocretacea Zone of the Late Cenomanian-Early Turonian and consists of calcareous shale and limestone. The calcareous shale facies is characterized by chlorite, smectite and illite with high concentration of P2O5, Zn, Ni, V, and Cr. The carbonate rocks are characterized by foraminiferal wakestone and bioclastic grainstone. These microfacies associations are characterized by barite with high content of Sr. The facies types with faunal contents, geochemical and mineralogical studies suggest a restricted to shelf lagoon open circulation environment. 相似文献
Efficient CO2 capture capabilities of activated carbons prepared from natural coal are presented. The preparation method involved simple chemical activation using wet impregnation or dry physical mixing of the raw sample with activating agents like KOH or NaOH. The activated materials were characterized for their structural and textural properties by different analysis techniques. The activated samples exhibited well‐developed porosity, large surface area, and high pore volumes and had other active elements like oxygenated functional groups. These groups modified the surface energy of the resultant samples. The superior performance of the activated carbons was attributed to several factors, including large surface area, presence of narrow micropores, and oxygenated functional groups on the surface. 相似文献
In this paper, we introduce a novel design for high performance silicon-based Fabry?CPérot cavities and their corresponding design model. According to the design model, the new design shows higher stability, lower insertion loss and higher quality factor Q. Our methodology was based, on one hand, on taking advantage of light reflection and refraction over curved surfaces with curvatures along 2 orthogonal directions, in order to confine the Gaussian beam inside the cavity, thus reducing loss due to beam divergence. Such design enables approaching new limits, where Q-factor is mainly governed by the mirrors reflectance. On the other hand, the use of Bragg reflectors, obtained by DRIE etching enables reaching reflectances above 99%, thus enabling very high Q-factors. 相似文献
The motion of a drop along a fibre is studied to formulate a method by which adhesives could be distributed within fibre bundles. The drops had to be located at the cross-points of fibres to provide strength and flexibility to the mat. Among the various methods of application, described in detail, only the solution method gave satisfactory results. An empirical relationship was derived to estimate the mass of the adhesives retained by the fibres. 相似文献
Training sequence is used in multiple antenna systems to estimate channel state information and mitigate channel distortion between transmitter and receiver. However, the training sequence or pilot must be limited to a certain size in order to reduce the impact of overhead loss due to limited channel coherence length in mobile users. In this paper, we proposed to use training size optimization in cell-free massive MIMO system. In addition, we proposed and compared the performance of different training size optimization algorithms, namely exhaustive search optimization, bisection optimization and min–max optimization, with each method has different level of calculation complexities. The results showed that in general, all of the 3 training length optimization methods improved the downlink rate compared to the conventional pilot length method. We also showed that the training optimization methods are more effective when the coherence length is small or the number of users is very large. In the case of large number of users or small coherence length, the exhaustive search has the best median downlink rate, followed closely by min–max optimum and finally the bisection method. Even though the exhaustive search optimization has the best downlink rate, we showed that the proposed reduce optimization complexity methods has significantly less calculation complexity. In addition, the median downlink rate performance of min–max optimization method is only slightly less than that of the exhaustive search method for various number of users and coherence length.
In this paper, we propose a learning assessment method based on the analysis of learner’s behavioural style. This method was first applied for wheel-chair driving tasks because it is simple and risk-free, but unusual for users. Seven classic performance indicators based on joystick control were used to characterise the users’ driving style. We assumed that the learning effectiveness of the users can be evaluated by comparing their driving style with the reference ones, which could be extracted from experienced users. The evaluation was carried out for six novice users and eight experienced users. The users were asked to carry out several typical driving tasks for seven trials at first. The fuzzy C-means clustering method was used with the data of the experienced users to obtain the reference driving styles. Next, an evaluation was performed for novice users by comparing their driving styles with the reference ones. The results showed that, for all of the experienced users, their driving styles could be classified into two reference types. In addition, there was no significant difference in their driving styles from one trial to another, even for a user with disabilities, which means that their driving style was stable. On the other hand, novice users had switching behaviours during the learning phase; however, after eight additional trials, each novice user’s driving style converged to one of the two identified reference types, meaning that the novice users could achieve a stable performance after learning, which was also validated by an expert therapist. 相似文献